CompoundsActive IngredientsHygromycin B CAS 31282-04-9

SKU: WGP-31282-04-9

Hygromycin B

CAS:31282-04-9

Molecular Formula: C20H37N3O13

Molecular Weight: 527.52

MOQ:1KG

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Description

Hygromycin B CAS 31282-04-9 Product Information

Product Name: Hygromycin B
Synonyms: antihelmycin;hydromycinb;hygromix2.4;hygromix-8;hygrovetine;Hygromycin Solution;Hygromix;5-O-(2,3-O-(6-Amino-6-desoxyheptapyranosyliden)-beta-D-talopyranosyl)-desoxy-N’-methyl-D-streptamin
CAS NO: 31282-04-9
Molecular Weight: 527.52
Molecular Formula: C20H37N3O13
Boiling Point: 608.13°C (rough estimate)
Melting point: 160-180°C
Density: 1.4252 (rough estimate)
Appearance: faintly brown to brown powder
Applications: Hygromycin B, a selective antibiotic effective against most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome
Solubility: H2O: 50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days.
Storage: 2-8°C

What is Hygromycin B CAS 31282-04-9

Made by the bacterium *streptomyces hygroscopicus*, hygromycin B, with a CAS number 31282-04-9, is an aminoglycoside antibiotic Its capacity to choose and preserve cells transformed with hygromycin B resistant genes makes it rather popular in molecular biology and genetic engineering. These are the main salient features of Hygromycin B:

1. Chemical Structure and Formula:

  • Hygromycin B’s molecular structure and formula are C20H37N3O13.
  • Its complicated structure, which is typical of aminoglycoside antibiotics, consists of many sugar moieties joined to a core aminocyclitol.

2. Physical Attributes:

  • Usually white to off-white powder, hygromycin B
  • Solubility: At neutral pH it produces a stable solution; it is soluble in water.

3. Pharmacological Properties:

  • By attaching to the 30S component of the bacterial ribosome, hygromycin B reduces translation and hence suppresses protein production.
  • It works against higher eukaryotic cells, fungus, and bacteria as well as a wide spectrum of prokaryotic and eukaryotic cells.

4. Applications in Molecular Biology:

  • Commonly utilised as a selection agent in genetic engineering and cell culture, hygromycin B finds applications in molecular biology. Whereas non-transformed cells are killed, successfully transformed cells using a vector encoding the hygromycin resistance gene (hygR) can survive and multiply in the presence of hygromycin B.
  • In mammalian, plant, and yeast cells, b. Gene Expression Studies helps to sustain and choose for stable transfections.
  • In biotechnological uses, hygromycin B is chosen to select for cells producing recombinant proteins.

5. Dosage and Usage:

  • The type of cell and the experimental environment affect the effective concentration of hygromycin B. For eukaryotic cells, usual values fall between 50 and 400 µg/mL; for prokaryotic cells, they fall between 20 and 200 µg/mL.
  • Before use, it is crucial to ascertain the minimum inhibitory concentration (MIC) for any particular cell line or organism.

6. Safety and Handling:

  • Upon exposure, hygromycin B can irritate the skin, eyes, and respiratory tract, so posing health hazards. If taken orally or breathed, it could also be dangerous.
  • When handling hygromycin B, appropriate PPE including gloves, lab coats, and eye protection should be worn. Work should be done in a fume hood or well-ventilated space.
  • Hygromycin B should be kept cold, dry, free from light and moisture. Solutions should be kept stable by refrigerated at -20°C.

7. To reduce environmental pollution, hygromycin B should be handled and disposed of in conformity with local rules and recommendations. It shouldn’t be let into the surroundings unprocessed.

All things considered, Hygromycin B is a flexible antibiotic mostly used in molecular biology for the selection and upkeep of genetically altered cells. In genetic engineering and biotechnological research, its broad-spectrum activity and efficiency in reducing protein synthesis make it an important instrument. Safe and efficient use of it depends on proper handling and safety precautions.

Transit time You will normally receive your parcel within 7-15 working days after shipment (this may be delayed in special circumstances, such as Chinese New Year).
Receiving method Generally we will send the goods by courier or special line, of course, if the goods themselves in the local warehouse have goods, also support self-pickup, depending on the circumstances.
Overseas warehouse We have overseas warehouses in some European countries and Australia, such as Germany, Russia and Australia.
Delivery Method WGP will ship via courier companies such as DHL, FedEx, UPS, TNT or EMS.
About After Sales Within 7 days of your receipt of the goods if you find any problems with the goods (broken packaging, less hair, etc.) please feel free to contact our sales, we will help you deal with it in time.

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